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1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides residue. The coupling antigens are pre-coated on the micro-well stripes. The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample. This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.4 ppb

Incubator temperature: 25℃

Incubator time: 60min20min

Detection limit

Tissue (high-detection-limit method)............................................... 0.4 ppb

Tissue (lower-detection-limit method)……………………………………...2 ppb

Honey, egg …………………………………………………………………0.4 ppb

Serum, urine................................................................................. 1.6 ppb

Milk................................................................................................. 8 ppb

Recovery rate

Tissue, honey............................................................................. 85±25%

Urine, milk, serum........................................................................ 70±20%

Cross-reaction rate

Sulfadiazine (SD or SDZ)................................................................. 100%

Sulfamonomethoxine (SMM)........................................................... 97.6%

Sulfamethoxazole (SMZ).................................................................. 100%

Sulfathiazole (ST).......................................................................... 195.0%

Sulfamerazine (SM1)........................................................................... 92%

(PST)................................................................. 73%

Sulfamethyltiadiazolum..................................................................... 110%

Sulfapyridine..................................................................................... 48%

Sulfamethoxydiazine(SMD)............................................................. 26.4%

Sulfafurazole(SIZ)........................................................................... 95.0%

Sulfanethazine(SM2)………………………………………………………. 140%

Sulfadimethoxine(SDM)…………………………………………………… 110%

Sulfadoxine (SDM2)…………………………………………………………..80%

Sulfaquinoxaline(SQX)………………………………………………………160%

Sulfamethoxypyridazine (SMP)………………………………………………80%

Sulfachlorinepyridazine (SPDZ)……………………………………………...75%

Sulfabenzoyl (SML)…………………………………………………………..104%

Conclusion drawn from the cross-reaction rate: This kit can be used for the test of Sulfonamides, totally meet the national testing demand of Sulfonamides.

3. Components

1) Micro-well strips: 12 strips with 8 removable wells each

2) 6× standard solution (1 mL each): 0 ppb, 0.4 ppb, 1.2 ppb, 3.6 ppb, 10.8 ppb and 32.4 ppb

3) Enzyme conjugate (7 mL)............................................................... red cap

4) Antibody working solution (10 mL)................................................ blue cap

5) Substrate A solution (7 mL)......................................................... white cap

6) Substrate B solution (7 mL)......................................................... black cap

7) Stop solution (7 mL).................................................................. yellow cap

8) 20× concentrated washing buffer (40 mL)..................................... white cap

9) 2× concentrated redissolving solution (50 mL)..................... transparent cap

4. Materials required but not provided

1) Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, votex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g)

2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30-300 µL;

3) Reagents: Acetonitrile (CH3CN), ethyl acetate, N-hexane, Na2HPO4·12H2O, NaH2PO4·2H2O, NaOH, HCl, Methylene chloride

5. Sample pre-treatment

Instructions (The following points must be dealt with before the pre-treatment)

1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;

2) Before the experiment, each experimental utensil must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.

Solution preparation before sample pre-treatment:

1) 0.2 M NaOH: dissolve 0.8 g NaOH in deionized water to 100 mL.

2) 0.5 M HCl (for honey): dissolve 4.3 mL HCI (36%) in deionized water to 100 mL.

3) 0.02 M PB buffer: dissolve 5.16g Na2HPO4·12H2O and 0.87g NaH2PO4·2H2O in the deionized water to 1L.

4) Acetonitrile (CH3CN) -Methylene chloride mixing solution

Vacetonitrile-Vmethylene chloride = 1 : 4

5) The 2× concentrated redissolving solution is diluted with deionized water at 1:1 (1 mL concentrated redissolbing solution + 1 mL deionized water), used for the treated sample redissolving.

5.1 Tissues (chicken, duck, pork/liver, egg, fish, shrimp etc.)

A. High-detection-limit method

Method one

1) Weigh 2.0 ± 0.05 g of the homogenized tissue sample into 50 ml centrifuge tube

2) Add Acetonitrile (CH3CN) -Methylene chloride mixing solution 8 ml, shake for 2 min, centrifuge at above 4000 r/min at 15 ℃for 10 min

3) Take 4 ml the clear organic phase (upper layer) into a dry tube, blow to dry with nitrogen or air completely by rotary evaporation at 50-60 ℃

4) Dissolve the dry residues in 1 mL of the diluted redissolving solution, add 1 mL N-hexane, shake vigorously for 30 seconds; centrifuge at above 4000 r/min at 15℃ for 5 min.

5) Remove the upper layer, take 20µl lower layer solution for further analysis.

Fold of dilution of the sample: 1

Method two

1) Weigh 3 ± 0.05 g of the homogenized sample, put into centrifugal tube, add 3 mL 0.02 M PB buffer, shake properly. Then add 4 mL ethyl acetate and 2 mL Acetonitrile (CH3CN), shake properly for 10 min, centrifuge at above 4000 r/min at 15 ℃ for 10 min;

2) Transfer 2 mL supernatant(approx 1 g sample) into a new centrifugal tube, blow to dry with nitrogen or air completely by rotary evaporation at 50-60 ℃

3) Add 1 mL N-hexane, then add 1 mL of the diluted redissolving solution, shake strongly for 30 s. Centrifuge at 4000 r/min at room temperature for 5 min, remove the upper layer

4)

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Posted by Samara Duan
e-MailContact company
Company
Shenzhen Biokau Sci-tech Co.,ltd
Address Rm206, Building A DongJin Block, Zhongshan Road,
City & ZIP
Shenzhen 518118
Country
China
Phone
For registered Members only.
Fax
For registered Members only.
Key Customers / Clientele Global
Company Profile We are a team of experienced scientists with many years of experience on antigen, antibody, Gold Colloid and ELISA kit.

We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine. BIOKAU has been supporting and inspiring the life science research community for over 5 years with products from hundreds of domestic and international manufacturers. We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.

BIOKAU provides the most up-to-date products and technical information available from world-class manufacturers, to laboratories, research institutes, life-science education and testing organizations around the world. We are proud that our ability to supply a vast array of products and information allows us to serve the needs of all of our customers. By making full use of our international network, coupled with the highest quality information, we support the life sciences community with a level of commitment and responsibility befitting a company that is trusted by its valued customers.
email:sales@biokau.com
URLFor registered Members only.
Rating (0 reviews)
Keywords (seperated by comma)Sulfonamides
Product SpecificationsSensitivity: 0.4 ppb
Incubator temperature: 25℃
Incubator time: 60min~20min
Detection limit
Tissue (high-detection-limit method)............................................... 0.4 ppb
Tissue (lower-detection-limit method)...2 ppb
Honey, egg 0.4 ppb
Serum, urine................................................................................. 1.6 ppb
Milk................................................................................................. 8 ppb
Recovery rate
Tissue, honey............................................................................. 8525%
Urine, milk, serum........................................................................ 7020%
Cross-reaction rate
Sulfadiazine (SD or SDZ)................................................................. 100%
Sulfamonomethoxine (SMM)........................................................... 97.6%
Sulfamethoxazole (SMZ).................................................................. 100%
Sulfathiazole (ST).......................................................................... 195.0%
Sulfamerazine (SM1)........................................................................... 92%
Phthalylsulfathiazole (PST)................................................................. 73%
Sulfamethyltiadiazolum..................................................................... 110%
Sulfapyridine..................................................................................... 48%
Sulfamethoxydiazine(SMD)............................................................. 26.4%
Sulfafurazole(SIZ)........................................................................... 95.0%
Sulfanethazine(SM2). 140%
Sulfadimethoxine(SDM) 110%
Sulfadoxine (SDM2)..80%
Sulfaquinoxaline(SQX)160%
Sulfamethoxypyridazine (SMP)80%
Sulfachlorinepyridazine (SPDZ)...75%
Sulfabenzoyl (SML)..104%
Conclusion drawn from the cross-reaction rate: This kit can be used for the test of Sulfonamides, totally meet the national testing demand of Sulfonamides.
Industry CategoryBio-Chemicals
Quality/Safety Certifications
Delivery Lead Time5days
Payment & Price TermsT/T
My Offers10% discount off
Product Portfolio / Other Products
CustomersGlobal
Detailed Company Introduction
FOB Price & Port250USD/test
Packaging Details96 wells/kit
Our TeamShenzhen Biokau Sci-tech Co.,ltd
Web SiteFor registered Members only.

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 Product Details
 Product id  389723
 Visits   1410
 Date  2015.05.02
 


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